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The Aging Landscape by scRNAseq of Mesenchymal Lineage Cells in Mouse Bone

5 months 3 weeks ago
A decrease in osteoblast number and bone formation are seminal contributors to age-related osteoporosis. However, the aging-associated molecular mechanisms that impact osteoblast precursors, osteoblasts, osteocytes, and other bone mesenchymal cell types remain unclear. We performed single-cell RNA-sequencing of mesenchymal cells present at the endosteum and periosteum of young and old C57BL/6 mice of both sexes. Osteoblast precursors and osteoblasts from female endosteum exhibited the greatest...
Melda Onal

Six Drivers of Aging Identified Among Genes Differentially Expressed With Age

5 months 3 weeks ago
Many studies have compared gene expression in young and old samples to gain insights on aging, the primary risk factor for most chronic diseases. However, these studies only identify associations without distinguishing drivers of aging from compensatory geroprotective responses or incidental downstream effects. Here, we introduce a workflow to characterize causal effects of differentially expressed genes on lifespan. First, we performed a meta-analysis of 25 gene expression datasets comprising...
Ariella Coler-Reilly

Preservation of Autophagy May Be a Mechanism Behind Healthy Aging

5 months 3 weeks ago
Autophagy is intricately linked with protective cellular processes, including mitochondrial function, proteostasis, and cellular senescence. Animal studies have indicated that autophagy becomes dysfunctional with aging and may contribute to T cell immunosenescence. In humans, it remains unclear whether autophagy is impaired in CD4^(+) T cells as people age. To answer this question, we examined basal and inducible autophagic activity in a series of experiments comparing CD4^(+) T cells from...
Arsun Bektas

Lactate Secretion by Monocytes as a Determinant of Innate Immune Cell Fitness in Healthy Elderly

5 months 3 weeks ago
Immune cell metabolism is increasingly recognized as an important regulator of immune function, but its role in age-related immune dysfunction, chronic inflammation, and cardiometabolic complications in humans remains incompletely understood. This study investigated the impact of aging on monocyte metabolic and functional signatures in a healthy elderly population. We aimed to leverage these immunometabolic signatures to identify healthy elderly individuals with reduced immune cell fitness and,...
Lisa Smeehuijzen

Analysis of EEG coherence according to the onset of Alzheimer's disease

5 months 3 weeks ago
Alzheimer's disease (AD) is one of the most common neurodegenerative diseases, the effects of which can be slowed down by an early and correct diagnosis. Greater diagnostic accuracy depends on a better understanding of the disease. In this regard, electroencephalography is a promising low-cost tool to explore brain activity and support the diagnosis of AD. When analyzing EEG signals, age is a factor that is not usually taken into account despite being one of the main risk factors. In this work,...
Mariana Escobar-López