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Low-Intensity Pulsed Ultrasound Treatment Selectively Stimulates Senescent Cells to Promote SASP Factors for Immune Cell Recruitment

1 year 5 months ago
As emerging therapeutic strategies for aging and age-associated diseases, various biochemical approaches have been developed to selectively remove senescent cells, but how physical stimulus influences senescent cells and its possible application in senolytic therapy has not been reported yet. Here we developed a physical method to selectively stimulate senescent cells via low-intensity pulsed ultrasound (LIPUS) treatment. LIPUS stimulation did not affect the cell cycle, but selectively enhanced...
HyeRan Gwak

Characterization of Human Senescent Cell Biomarkers for Clinical Trials

1 year 5 months ago
There is an increasing need for biomarkers of senescent cell burden to facilitate the selection of participants for clinical trials. p16^(Ink4a) is encoded by the CDKN2A locus, which produces five variant transcripts in humans, two of which encode homologous p16 proteins: p16^(Inka4a), encoded by p16_variant 1, and p16ɣ, encoded by p16_variant 5. While distinct quantitative polymerase chain reaction primers can be designed for p16_variant 5, primers for p16_variant 1 also measure p16_variant 5...
Joshua N Farr

Deciphering transcription activity of mammalian early embryos unveils on/off of zygotic genome activation by protein translation/degradation

1 year 5 months ago
Quantification of transcription activities in mammalian preimplantation embryos is challenging due to a huge amount of maternally stored transcripts and paucity of research materials. Here, we investigate genome-wide transcription activities of mouse and human preimplantation embryos by quantifying elongating RNA polymerase II. Two transcriptional waves are identified in early mouse embryos, with summits at the 2-cell and 8-cell stages. Gene collections with different expression patterns are...
Yi-Ran Zhang

Distinct myeloid-derived suppressor cell populations in human glioblastoma

1 year 5 months ago
Science, Volume 387, Issue 6731, January 2025.
Christina Jackson, Christopher Cherry, Sadhana Bom, Arbor G. Dykema, Rulin Wang, Elizabeth Thompson, Ming Zhang, Runzhe Li, Zhicheng Ji, Wenpin Hou, Wentao Zhan, Hao Zhang, John Choi, Ajay Vaghasia, Landon Hansen, William Wang, Brandon Bergsneider, Kate…