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Age-associated metabolic and epigenetic barriers during direct reprogramming of mouse fibroblasts into induced cardiomyocytes

10 months 1 week ago
Heart disease is the leading cause of mortality in developed countries, and novel regenerative procedures are warranted. Direct cardiac conversion (DCC) of adult fibroblasts can create induced cardiomyocytes (iCMs) for gene and cell-based heart therapy, and in addition to holding great promise, still lacks effectiveness as metabolic and age-associated barriers remain elusive. Here, by employing MGT (Mef2c, Gata4, Tbx5) transduction of mouse embryonic fibroblasts (MEFs) and adult (dermal and...
Francisco Santos

Exploring the perceptions and experiences of older people on the use of digital technologies during the COVID-19 pandemic: a qualitative study

10 months 1 week ago
CONCLUSION: Digital technologies offered a lifeline during COVID-19 to maintain social contact and their use was found acceptable by older people. Digital platforms such as Zoom can be further employed to conduct remotely delivered interventions with the aim to increase uptake of social and physical activity interventions within this population.
Lesley-Anne Tanhamira

Sleep deprivation leads to non-adaptive alterations in sleep microarchitecture and amyloid-β accumulation in a murine Alzheimer model

10 months 1 week ago
Impaired sleep is a common aspect of aging and often precedes the onset of Alzheimer's disease. Here, we compare the effects of sleep deprivation in young wild-type mice and their APP/PS1 littermates, a murine model of Alzheimer's disease. After 7 h of sleep deprivation, both genotypes exhibit an increase in EEG slow-wave activity. However, only the wild-type mice demonstrate an increase in the power of infraslow norepinephrine oscillations, which are characteristic of healthy non-rapid eye...
Neža Cankar

The immune landscape of murine skeletal muscle regeneration and aging

10 months 1 week ago
Age-related alterations in the immune system are starting to emerge as key contributors to impairments found in aged organs. A decline in regenerative capacity is a hallmark of tissue aging; however, the contribution of immune aging to regenerative failure is just starting to be explored. Here, we apply a strategy combining single-cell RNA sequencing with flow cytometry, histological analysis, and functional assays to perform a complete analysis of the immune environment of the aged regenerating...
Neuza S Sousa

In Science Journals

10 months 1 week ago
Science, Volume 386, Issue 6723, Page 740-742, November 2024.

Michael Funk, and
Bianca Lopez,
Keith T. Smith,
Brent Grocholski,
Mattia Maroso,
Jake S. Yeston,
Jesse Smith,
Stella M. Hurtley,
Courtney Malo,
Michael A. Funk,
Di Jiang,
L. Bryan Ray,
Ankit Walia,
Phil Szuromi,
Ian S. Osborne,
Hannah M…