Aging & Longevity

No abstract

Intestinal stem cells (ISCs) drive the rapid regeneration of the gut epithelium. However, during aging, their regenerative capacity wanes, possibly through senescence and chronic inflammation, albeit little is known about how aging-associated dysfunction arises in the intestine. We previously identified the urokinase plasminogen activator receptor (uPAR) as a senescence-associated protein and developed CAR T cells able to efficiently target it. Harnessing them, here, we identify the accumulation...

Aging exacerbates organ injury in endotoxemia, but it is not clear whether endotoxemia is associated with a specific, age-related profile of the endothelial response. Therefore, the aim of the study was to assess the pattern of endothelial response to lipopolysaccharide (LPS) in aged mice (18-month-old) as compared to young mice (3-month-old). Our analysis was based on functional endothelial responses measured in vivo by magnetic resonance imaging (MRI) and on a comprehensive panel of biomarkers...

Arterial aging is associated with enhanced angiotensin II (Ang II) signaling via Ang II type 1 receptor (AT1R) and with microRNA-34a (miR-34a) increased expression. AT1R-associated protein (ATRAP/Agtrap) binds to AT1R, promotes its internalization, and inhibits Ang II signaling. This study addresses the hypothesis that miR-34a targets ATRAP/Agtrap and enhances Ang II pro-inflammatory signaling via AT1R in arterial vascular smooth muscle cells (VSMC). Our results show that miR-34a exhibits an...

CONCLUSION: Differences in state-level health system capacity may disproportionately affect socioeconomically disadvantaged populations, underscoring the need for more equitable hypertension management and cardiovascular health strategies across India.

CONCLUSIONS: Relationships between joint pain and poor sleep quality among older adults with radiographic knee OA were similar, regardless of physical activity level. Our results highlight the high prevalence of both sleep disturbance and significant knee pain in this group, illustrating the need to consider supportive measures as appropriate in this population.

With the aging population in Europe, particularly Hungary, unhealthy aging is emerging as a growing public health challenge. Physical inactivity is a modifiable risk factor for age-related diseases and remains highly prevalent. Increasing daily physical activity is a key strategy for extending health span. Step-count-based interventions, including motivational interviewing and email-based feedback, have been shown to promote physical activity, but direct comparisons of these approaches in...

Ageing dampens the regenerative potential of intestinal epithelium across species including humans, yet the underlying causes remain elusive. Here we characterized the temporal dynamics of regeneration following injury induced by 5-fluorouracil, a commonly used chemotherapeutic agent, using proteomic and metabolomic profiling of intestinal tissues together with functional assays. The comparison of regeneration dynamics in mice of different ages revealed the emergence of proteostasis stress and...

Alzheimer's disease causes progressive cognitive decline, yet some individuals remain resilient despite developing hallmark pathology. A subset of people with Down syndrome (DS), the most common genetic cause of Alzheimer's disease, demonstrates such resilience. Given the elevated risk of hematopoietic mutations in DS, we hypothesize that certain variants may confer microglial resilience. Here, we introduce a myeloid DS-linked CSF2RB A455D mutation into human pluripotent stem cell-derived...

Biomechanical alterations contribute to the decreased regenerative capacity of hematopoietic stem cells (HSCs) upon aging. RhoA is a key regulator of mechanosignaling, but its role in mechanotransduction in stem cell aging remains unclear. Here we show that murine HSCs respond to increased nuclear envelope (NE) tension by inducing NE translocation of P-cPLA2, which cell-intrinsically activates RhoA. Aged HSCs experience physiologically higher intrinsic NE tension, but reducing RhoA activity...

CONCLUSIONS: Unexpected stopping highlights age-related changes in movement strategy. Older adults shift from a distal-driven braking to proximal, multi-joint co-contraction strategy, characterized by increased hip work, reduced knee work, and greater muscle synergy complexity. This proximal engagement reflects a tradeoff along the stability-efficiency continuum: it enhances safety but increases metabolic cost and postural sway. By disrupting the distal-to-proximal control sequence, this...

Aging and diabetes mellitus (DM) are associated with declines in physical fitness, such as reduced muscle strength, balance, and flexibility, increasing the risk of falls and functional limitations. This pilot study used a quasi-experimental design with pre- and post-intervention assessments to evaluate the effects of a 6-month sensorimotor training (SMT) program on physical fitness in older women with type 2 diabetes. Ten women aged 65 and older were divided into an intervention group (IG, n =...

CONCLUSION: Our study reveals volatility in case numbers post-SARS-CoV-2, with males disproportionately affected. A high proportion of elderly patients take anticoagulants. Among the injured, there is a high rate with cerebral injury. Most fractures occur in exposed facial regions. Ongoing surveillance is essential to address increasing geriatric vulnerability.

CONCLUSIONS: Older adults' perceptions of health-promoting activities were influenced by personal, inter-personal and environmental factors. Contextualizing lifestyle modification programs that address their preferences and real-life situations may facilitate engagement. Older adults may also benefit from future initiatives that empower them to choose among feasible options to increase their self-efficacy for preventing frailty.

CONCLUSIONS: The results of the concept analysis of relocation in older adults revealed a comprehensive insight into this concept. Successful relocation in the older adults is achieved by considering the physical, personal, social, economic, and psychological aspects, and the preparation and planning of the older adults.

The elimination of senescent cells can enhance the osteointegration of implants in elderly patients. However, achieving specific clearance of senescent cells without adversely affecting the function of normal cells remains challenging. Here we show an implant surface modification technique to achieve specific clearance of locally senescent cells by modulating their metabolism. Our method involve modifying implants with BPTES, a glutaminase 1 (GLS1) inhibitor, through π-π stacking with dopamine....

While coastal renewable energy-powered seawater electrolysis is highly promising for green H(2) production, the anodic chemical corrosion by aggressive chlorine chemistry and violent bubble release-induced physical damage to anodes are two long-standing issues that lead to inferior stability. Here we pursue integrating triple protection to a monolithic catalyst to concurrently alleviate chlorine chemistry and weaken external forces from bubble escaping/collapsing. The 1^(st) and 2^(nd) defenses...

As a powerful oxidant, atomic oxygen (O) holds considerable promise for a variety of biomedical and industrial applications. However, the inability to quantify solvated oxygen atoms has prevented the determination of the fundamental parameters governing its behaviour in relevant aqueous environments. Here, we directly image ground-state oxygen atoms in water using femtosecond two-photon absorption laser-induced fluorescence. Measurements show that oxygen atoms persist for tens of microseconds in...

Mammalian maximum lifespan (MLS) varies over a hundred-fold, yet the molecular mechanisms underlying this diversity remain unclear. We present a cross-species analysis of alternative splicing (AS) across six tissues in 26 mammals, identifying hundreds of conserved AS events significantly associated with MLS, with the brain containing twice as many tissue-specific events as peripheral tissues. MLS-AS events are enriched in pathways related to mRNA processing, stress response, neuronal functions,...

Age-associated decline in tissue NAD^(+) levels contribute to functional impairments, recognized as aging. Nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme crucial for NAD^(+) biosynthesis in mammals, is encapsulated in extracellular vesicles (EVs) and secreted into the bloodstream. The importance of extracellular NAMPT-containing EVs (eNAMPT-EVs) in hypothalamic NAD^(+) biosynthesis has been demonstrated in several mouse models. However, whether eNAMPT-EVs derived from...