Aging & Longevity

INTRODUCTION: MicroRNAs (miRNAs) are promising biomarkers for the diagnosis and prognosis of neurodegenerative diseases. Over the past few years, miR-34a-5p and miR-126-3p have become some of the most characterized miRNA, the former being associated with cellular senescence and apoptosis and the latter with the maintenance of vascular endothelial function. The present study aimed to evaluate diagnostic performance of miR-34a-5p and miR-126-3p for cognitive dysfunction in CSVD patients. In...

Mild cognitive impairment (MCI) is associated with age-related alterations in neural oscillatory activity and cerebral hemodynamics, while mobility limitations often restrict participation in conventional exercise-based interventions. This study investigated exploratory, single-arm, within-subject short-term neurovascular and electrophysiological responses to a combined visual and vibration stimulation protocol in older adults with MCI. Cerebral blood flow was assessed using near-infrared...

Chronic pain affects more than half of older adults and has been increasingly linked to cognitive decline, a relationship with profound implications for healthy aging. Ramezani and colleagues examined this association in 1,343 Iranian older adults from the Birjand Longitudinal Aging Study, a well‑characterised cohort from an understudied population. The study has notable strengths: a large sample size, population‑based recruitment, and DAG‑guided covariate selection. However, three...

CONCLUSIONS: Structural and functional muscle indicators showed divergent age-related patterns among rural community-dwelling women. The performance-based 5×STS demonstrated substantially greater relative differences than structural indicators, supporting the inclusion of functional assessments alongside structural measures for age-stratified evaluation in rural settings.

Anti-aging pharmacology has transitioned from early exploratory lifespan-extension studies to a hallmark-informed, multi-level framework that integrates mechanistic, preclinical, and translational evidence. Using a bibliometric-guided, strategy-oriented approach, this review maps the explicit anti-aging drug literature from 2005 to 2025, identifies historically influential compounds, and evaluates their translational readiness. The field converges on three partially overlapping intervention...

CONCLUSIONS: The findings from our study indicate that supporting the psychological and spiritual resources of older adults plays an important role in strengthening the successful aging process. Longitudinal studies on successful aging are recommended.

Aging is associated with structural and functional changes of the vocal folds that may result in presbyphonia, often perceived as a weak or shaky voice. However, the quantitative characterization of underlying age-related vocal tremor across the adult lifespan remains limited. This cross-sectional study investigated the characteristics of vocal tremor across the adult lifespan using automated acoustic analysis. A total of 291 native speakers aged 18-94 years were recruited and underwent...

Hippocampal atrophy across the lifespan is associated with cognitive decline, as is difficulty understanding speech-in-noise. The hippocampus contains subfields with distinct functions, but their involvement in hearing-related differences is unknown. Here, we used HippUnfold, an automated hippocampal unfolding and subfield segmentation method, together with Multiple Factor Analysis (MFA) to examine shared variance across hippocampal structure, hearing-related function, and cognition in younger...

Aging disrupts physiological homeostasis, impairing thermoregulation, metabolism, and water balance, but the underlying neural mechanisms remain unclear. Here, we identify arginine vasopressin (AVP) neurons in the supraoptic nucleus (SON) of the hypothalamus as a critical driver of these changes in male mice. Single-nucleus RNA-sequencing revealed Avp among the most upregulated neuronal transcripts with age. Aged SON^(AVP) neurons displayed enlarged size and heightened excitability, features...

The goal of developing interventions to slow ageing is not only lifespan extension but more importantly to increase healthspan, the period of life spent in active good health. Nutritional interventions have emerged as a potential strategy to maintain health with age. Testing these interventions for effects on human ageing would take several years and require large cohort sizes. We therefore employed C. elegans as a rapidly ageing model organism to investigate the effects of two commercially...

CONCLUSIONS: This study indicates that retaining ≥ 20 teeth, brushing at least once daily, and using dentures are associated with a lower likelihood of anxiety symptoms among the elderly population. Further prospective studies are warranted to confirm these associations.

CONCLUSION: Advancing age is strongly associated with an increased burden of multimorbidity, which is intricately linked to depressive symptoms, diminished HRQoL, reduced muscular strength, central adiposity, and physical inactivity. These findings highlight the urgent need for integrated interventions in institutionalised older adults in sub-Saharan Africa and should inform policy reform aimed at strengthening long-term care and healthy ageing strategies.

CONCLUSION: This study highlights how older adults' perceptions of peers' OA are shaped by interpersonal impressions and social norms. Charisma, warmth, and facial expression could reduce the importance of specific orofacial features, whereas missing teeth, visible poor oral health, or appearance changes perceived as outside age-appropriate norms could trigger assumptions about lifestyle, vulnerability, or self-care.

CONCLUSIONS: Pre-social frailty and social frailty are common health challenges faced by community-dwelling older adults in China. Recent data indicate that the prevalence of these conditions remains high; although the prevalence is the very high heterogeneity and should be interpreted with caution, it nevertheless underscores the necessity and urgency of implementing effective interventions. Early identification and intervention for individuals at risk of social frailty are of critical...

Japan has one of the world's longest life expectancies, yet biomarkers associated with disability and mortality in very old adults remain uncertain. The goal of this study was to identify plasma proteins associated with incident disability and mortality in community-dwelling octogenarians. Two prospective cohorts were analyzed: the Kawasaki Aging Well-being Project (KAWP; 2017-2018; 4.5-year follow-up) as discovery and Invecchiare in Chianti (InCHIANTI; 1998-2000; 15-year follow-up) for external...

Mitochondrial DNA (mtDNA) mutations are hallmarks of aging. mtDNA in all opisthokonts is replicated exclusively by DNA Polymerase γ (Pol γ; encoded by POLG). PolgD257A/D257A mice, lacking Pol γ exonuclease proofreading (exo-), exhibit premature aging and higher mtDNA mutation rates than Polgwt/wt (exo+) mice. Using short-read sequencing and the ultra-sensitive LostArc indel-junction detection pipeline, we analyzed mtDNA from exo- and exo+ mice across 10 tissues. Indel-junction frequency,...

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Accurate identification and quantification of senescence-modulating compounds require screening platforms that can distinguish between phenotypically distinct drug response profiles. Conventional approaches relying on single-parameter measurements-either cell viability or senescence markers alone-cannot differentiate senolytic-like or anti-aging-like response profiles from non-specific cytotoxicity or proliferative effects. Here, we present the quantitative Cellular AGing Evaluation system...

The NLRP3 inflammasome has been implicated in a wide range of human diseases, including cardiovascular, metabolic, neurodegenerative (such as Alzheimer's disease), and other age-related conditions. This has positioned NLRP3 as a promising pharmacological target. Numerous studies have shown that complete NLRP3 ablation can prevent or mitigate these diseases. However, total elimination of NLRP3 is not a feasible therapeutic strategy for the millions of patients affected by these degenerative...

Aging tissues lose function in part because stem cells change in number and behavior, but how age-related changes in the stem cell niche drive these processes is not well understood. Using the fruit fly testis, we asked how aging of the niche microenvironment influences stem cell maintenance and competition. We show that levels of niche cell-derived bone morphogenetic protein (BMP) signals decline with age, leading to increased expression of the transcriptional corepressor Hairless in germline...